ABCF3 (ATP binding cassette subfamily F member 3) is a member of the ABC transporter family that functions as an ATP-binding protein involved in cellular stress responses and potential antiviral activities. The protein contains functional Walker A/B motifs essential for ATP binding and demonstrates high-affinity binding to 2'-5' oligoadenylates, nucleotide second messengers involved in innate immune signaling 1. ABCF3 exhibits antiviral effects against flaviviruses such as West Nile virus when functioning in conjunction with OAS1B. The gene is highly expressed across various cancer types, including retinoblastoma, melanoma, and ovarian cancer 234. In ovarian cancer, downregulation of ABCF3 is associated with better chemotherapy sensitivity and longer time to progression 4. The protein's expression is regulated by multiple miRNAs and has been identified as a potential biomarker in oral cancer 5. ABCF3 can be successfully expressed and purified in yeast systems for biochemical studies 6. While the protein's interaction with 2'-5' oligoadenylates suggests involvement in antiviral signaling pathways, the complete biological consequences of these interactions require further investigation 1.