ABHD16B is a phospholipase and lipase enzyme that regulates cellular lipid homeostasis through coordinated lipid catabolism and synthesis. As a phosphatidylserine-specific phospholipase A1 (PS-PLA1), ABHD16B hydrolyzes phosphatidylserine at the sn-1 position with high specificity, mediated by electrostatic interactions between the PS headgroup and key residues (K8, R319, E178) that position the substrate at the catalytic site 1. The enzyme also functions as a monoacylglycerol lipase, preferentially hydrolyzing the 1-acylglycerol form of monoacylglycerols 1. Beyond direct lipid hydrolysis, ABHD16B plays a regulatory role in lipid homeostasis by modulating genes involved in neutral lipid degradation and phospholipid synthesis. Overexpression of ABHD16B decreases cellular triacylglycerol levels and increases phospholipid synthesis, with corresponding reductions in lipid droplet formation and modifications to fatty acid composition 2. These findings suggest ABHD16B coordinates a complex metabolic shift from neutral lipid storage toward membrane phospholipid synthesis. ABHD16B has been identified in epigenetic studies examining the effects of systemic corticosteroid exposure 3, though its clinical disease relevance remains to be fully elucidated.