ACOT11 (acyl-CoA thioesterase 11) is a mitochondrial enzyme that catalyzes hydrolysis of long-chain fatty acyl-CoA thioesters, particularly palmitoyl-CoA and myristoyl-CoA, to regulate intracellular fatty acyl-CoA concentrations 1. The enzyme contains steroidogenic acute regulatory related lipid transfer (START) domains that may function in lipid transfer and allosteric regulation of catalytic activity 2. ACOT11 is induced by cold exposure in brown adipose tissue, suggesting a role in thermogenic metabolic adaptation 3. Disease associations include involvement in neurodegeneration, where ACOT11 variants interact epistatically with APOE in Alzheimer's disease pathogenesis 4, and genetic variants associate with varicose veins 5. Clinically, elevated ACOT11 expression correlates with poor outcomes in lung cancer, including both squamous cell carcinoma and adenocarcinoma, where it functions as a prognosis-associated mitochondrial metabolism-related gene 678. ACOT11 expression remains stable under endoplasmic reticulum stress in lung cancer cells, distinguishing it from other acyl-CoA metabolic enzymes 8. These findings position ACOT11 as an important regulator of fatty acid metabolism with implications for metabolic homeostasis, neurodegeneration, and cancer progression.