ANKRD28 (ankyrin repeat domain 28) is a regulatory subunit of protein phosphatase 6 (PP6) that functions as a critical component of PP6 heterotrimer complexes 1. It serves as a scaffold protein that recognizes and facilitates dephosphorylation of specific substrates, including NFKBIE and MOB1, thereby modulating NF-κB signaling and focal adhesion dynamics 1. ANKRD28 interacts with DOCK180 to regulate cell migration through p130(Cas) phosphorylation and focal adhesion assembly, controlling the distribution of key migration proteins like Crk and paxillin 2. The protein also interacts with tumor suppressor BRCA1 in the cytoplasm, suggesting involvement in modulating PP6 signaling related to stress responses 3. In disease contexts, ANKRD28 has emerged as a significant factor in hematologic malignancies. Extrachromosomal circular DNA containing ANKRD28 (eccANKRD28) functions as an active enhancer driving multiple myeloma drug resistance through a POU2F2-mediated transcriptional network activating oncogenic pathways including IRF4 and BCL2 4. ANKRD28 is also identified as a super enhancer-associated gene implicated in germinal center B-cell diffuse large B-cell lymphoma prognosis, where its knockdown inhibits cancer cell proliferation 5. Additionally, ANKRD28-NUP98 fusion resulting from cryptic translocation t(3;5;11) has been associated with myelodysplastic syndrome and acute myelogenous leukemia 6.