ARL14EP (ARF like GTPase 14 effector protein) is a regulatory protein that functions in multiple cellular processes including vesicle trafficking, chr11 remodeling, and gene expression regulation. The protein interacts with ARL14 and MYO1E to connect MHC class II-containing cytoplasmic vesicles to the actin network, controlling vesicle movement along the actin cytoskeleton in dendritic cells [UniProt]. In epigenetic regulation, ARL14EP works with MET-2/SETDB1 and LIN-65/ATF7IP to prevent monoallelic expression by antagonizing histone methylation pathways 1. The gene shows significant disease relevance, particularly in polycystic ovary syndrome (PCOS), where increased ARL14EP expression is strongly associated with PCOS risk across multiple cohorts 2. ARL14EP expression is dynamically regulated during fetal development in gonadal, metabolic, and brain tissues, suggesting developmental roles in multiple organ systems 3. The gene is also associated with circadian light sensitivity, where variants enhance the effect of daylight exposure on chr11 4. Additionally, ARL14EP deletion may worsen lens abnormalities in WAGR syndrome patients 5. These findings indicate ARL14EP functions as a multifunctional regulatory protein with roles in cellular trafficking, chr11 regulation, and developmental processes across multiple tissues.