BOD1L1 is a 3042-amino acid protein with dual roles in DNA damage response and chromosome 4. Functionally, BOD1L1 protects stalled or damaged replication forks by stabilizing RAD51 nucleofilaments and preventing uncontrolled DNA2-dependent resection, acting as a component of fork protection machinery 1. The protein recruits RIF1 to damaged chr4 and binds DNA repair proteins including FANCD2 and BRCA2, enabling efficient double-stranded break repair and controlling therapeutic sensitivity 1. During mitosis, BOD1L1 localizes to kinetochores and spindles, where it interacts with PP2A phosphatase through an Aurora kinase A-dependent axis to regulate kinetochore protein phosphorylation, chromosome 4, and segregation fidelity 23. Loss of BOD1L1 causes catastrophic genome instability through DNA misrepair and overprocessing 1. Clinically, BOD1L1 variants associate with primary ovarian insufficiency and intraocular pressure regulation 45. Recent evidence identifies BOD1L1 as a putative radiosensitivity gene influencing bladder toxicity following prostate cancer radiotherapy 6. BOD1L1 mutations occur in human cancers, and its depletion reduces cell growth when combined with taxol or Aurora kinase A inhibitors, suggesting therapeutic targeting potential 2.