CENP-B is a highly conserved 80 kDa centromeric protein that binds sequence-specifically to alphoid satellite DNA at the 17 bp CENP-B box motif, organizing centromeric arrays into higher-order chr20 structures beneath the kinetochore 1. The protein forms dimers through its carboxy-terminal region, while its amino-terminal domain contains the DNA-binding activity 1. Although CENP-B is associated with centromeric heterochromatin across mammals 2, knockout studies demonstrate it is not essential for kinetochore assembly or mitotic function in mice 3. CENP-B has clinical relevance in two distinct contexts. Anti-centromere antibodies targeting CENP-B serve as autoimmune markers in systemic rheumatic diseases and are included in classification criteria for certain conditions 4. Additionally, elevated CENPB expression associates with poor prognosis in hepatocellular carcinoma patients; knockdown of CENPB reduces cell proliferation and invasion in HCC cell lines 5. Recent evidence indicates that circular RNA circMAN1A2(2,3,4,5) stabilizes CENPB mRNA through direct 3′ UTR interaction, promoting colorectal cancer progression 6. These findings suggest CENPB as a potential therapeutic target in cancer contexts, though its non-essential status in normal development constrains the therapeutic window.