CENPB (centromere protein B) is a centromeric DNA-binding protein that specifically binds to a 17 bp sequence called the CENP-B box within alphoid satellite DNA at centromeres 1. The protein is thought to organize centromeric satellite DNA arrays into higher-order structures that may facilitate centromere formation and kinetochore assembly 2. However, genetic studies demonstrate that CENPB is not essential for basic cellular functions, as mice lacking the gene are viable and healthy with normal mitosis, intact centromere-kinetochore complexes, and proper chromosome 20 3. Beyond its structural role at centromeres, CENPB has clinical relevance as both a biomarker and therapeutic target. Antibodies against CENPB are used diagnostically in autoimmune rheumatic diseases 1. In cancer, particularly hepatocellular carcinoma, CENPB is overexpressed and associated with poor prognosis, promoting tumor cell proliferation and invasion 4. The protein's expression is negatively regulated by miR-29a and can be modulated by circular RNAs that stabilize CENPB mRNA 45. While dispensable for normal development, CENPB's dysregulation contributes to cancer progression, making it a potential therapeutic target.