CEP250 encodes C-Nap1, a centrosomal protein essential for maintaining centrosome cohesion during interphase 12. The protein accumulates at centriole proximal ends and recruits CCDC102B, forming supramolecular assemblies with viscous properties that generate intercentriolar linkages 12. CEP250 plays critical roles in asymmetric cell division and stem cell maintenance; CEP250-deficient mice show premature centrosome separation leading to germ stem cell depletion and infertility 3. The protein is also involved in ciliogenesis and photoreceptor function 4. Loss-of-function CEP250 mutations cause nonsyndromic retinitis pigmentosa and progressive sensorineural hearing loss through centrosome localization defects and hair cell degeneration 45. Mutations in CEP250 are associated with atypical Usher syndrome presenting with combined retinal dystrophy and hearing loss 6, and acephalic spermatozoa syndrome affecting sperm head-tail connection 7. Clinically, CEP250 expression levels correlate with hepatocellular carcinoma and pancreatic cancer prognosis; high CEP250 expression associates with altered centrosome organization and poor patient outcomes 89. CEP250 represents a novel therapeutic target, with small-molecule inhibitors demonstrating potential antiviral activity against SARS-CoV-2 10.