CPT1B (carnitine palmitoyltransferase 1B) is a mitochondrial outer membrane enzyme that catalyzes the rate-limiting step of fatty acid oxidation by converting long-chain fatty acyl-CoA to acyl-carnitines for mitochondrial transport 1. This process is essential for cellular energy production, particularly in tissues with high metabolic demands. The CPT1 family includes three isoforms (CPT1A, CPT1B, CPT1C), with CPT1B being the muscle-specific isoform 1. CPT1B plays a critical role in metabolic reprogramming during cardiomyocyte proliferation, where altering CPT1B expression can promote adult cardiomyocyte proliferation by reversing metabolic changes 2. The enzyme's activity is regulated through post-translational modifications, including ubiquitination and succinylation, which can affect its stability and enzymatic function 34. Clinical significance includes its involvement in diabetic cardiomyopathy, where impaired fatty acid oxidation leads to cardiac lipotoxicity 4. Additionally, CPT1 deficiencies are associated with inborn errors of fatty acid oxidation, representing more than 15 described genetic defects that result in severe metabolic consequences 1. Understanding CPT1B regulation and function has therapeutic implications for metabolic disorders and cardiac diseases.