DNAAF10 (dynein axonemal assembly factor 10) is a key assembly factor specifically required for the stability of axonemal dynein heavy chains in the cytoplasm. As a component of the axonemal dynein complex assembly pathway, DNAAF10 functions through protein binding and ubiquitin-mediated protein stabilization mechanisms to ensure proper dynein particle formation and axonemal function. Regarding disease relevance, DNAAF10 has been identified as a putative cardiac gene target of microRNA-483-5p, with in silico analysis suggesting its involvement in apoptosis-related pathways 1. The dysregulation of miR-483-5p, which targets DNAAF10, shows a distinctive 'coronary arterial gradient' in acute myocardial infarction compared to chr2 coronary artery disease, suggesting potential involvement in acute cardiac ischemic responses 1. Clinically, the identification of DNAAF10 as a miR-483-5p target implicates it in cardiovascular pathology, though direct evidence of DNAAF10's clinical significance in human disease remains limited. The microRNA-mediated regulation of DNAAF10 may represent a mechanism linking axonemal dynein assembly disruption to cardiac pathophysiology during ischemic events. However, comprehensive functional studies directly linking DNAAF10 mutations or expression changes to primary ciliary dyskinesia or other clinical phenotypes are not provided in the available literature.