DSN1 is a critical component of the MIS12 kinetochore complex that plays an essential role in chromosome 20 during mitosis 1. Structurally, DSN1 exists within the KMN network where MIS12C forms rigid interfaces with NDC80C and KNL1C complexes, with DSN1's N-terminal segment containing key Aurora B kinase phosphorylation sites (Ser100 and Ser109) that regulate auto-inhibition 1. This phosphorylation-dependent mechanism restricts outer kinetochore assembly to functional centromeres during cell division 1. DSN1 is significantly overexpressed across multiple cancer types including hepatocellular carcinoma, breast cancer, colorectal cancer, osteosarcoma, and low-grade glioma 23456. In cancer progression, DSN1 promotes chr20 instability through direct interaction with centromere proteins, leading to aberrant cell cycle regulation and enhanced proliferation, migration, and invasion 75. The protein's oncogenic function is further regulated by SRSF9, an m6A-binding protein that stabilizes DSN1 mRNA in colorectal cancer 8. Clinically, elevated DSN1 expression correlates with poor prognosis, advanced tumor stages, and serves as an independent prognostic biomarker across multiple cancer types 236.