DYNC1I1 encodes dynein cytoplasmic 1 intermediate chain 1, a non-catalytic accessory component of the cytoplasmic dynein 1 complex that facilitates intracellular retrograde transport along microtubules 1. The protein mediates dynein binding to dynactin and cargo transport, playing essential roles in spindle organization and cytokinesis during meiosis through interactions with CLASP1 and PLK1 1. DYNC1I1 is involved in Lewy body pathology, as the protein colocalizes with phosphorylated α-synuclein in brainstem and cortical Lewy bodies in Parkinson's disease and dementia with Lewy bodies 2. The gene contains enhancer sequences in exons 15 and 17 that regulate limb development genes, with deletions causing split hand-split foot malformation and intellectual disability 3. DYNC1I1 demonstrates significant oncogenic potential, with overexpression associated with poor prognosis in multiple cancers including gastric cancer 4, head and neck squamous cell carcinoma 5, and hepatocellular carcinoma 67. In cancer contexts, DYNC1I1 promotes cell proliferation and inhibits apoptosis through various signaling pathways including AKT/ERK activation 7, establishing it as both a critical cellular transport component and potential therapeutic target.