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GeneE
10 sources retrieved · Most recent: April 2026 · Index updated 15 days ago
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EBAG9
estrogen receptor binding site associated antigen 9
Chromosome 8 · 8q23.2
NCBI Gene: 9166Ensembl: ENSG00000147654.17HGNC: HGNC:3123UniProt: O00559
106PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
protein bindingGolgi apparatussecretory granuleregulation of cell growthneoplasmoral squamous cell carcinomacancerisolated agammaglobulinemia
✦AI Summary

EBAG9 is an estrogen-responsive gene located at chromosome 8 that functions as a tumor-promoting factor with dual roles in cell death and immune evasion. Primary function: EBAG9 may participate in suppression of cell proliferation and induce apoptotic cell death through activation of ICE-like proteases 1. However, paradoxically, EBAG9 overexpression promotes tumor growth in vivo through its coiled-coil region, likely via intracellular associations with unidentified binding partners 2. Mechanism: EBAG9 is identical to RCAS1, a cancer cell surface antigen implicated in immune escape 1. The protein inhibits release of cytolytic enzymes from cytotoxic T lymphocytes and inversely correlates with intratumoral T cell infiltration 34. Disease relevance: EBAG9 is upregulated in ischemic acute kidney injury within injured proximal tubules displaying proapoptotic and proinflammatory phenotypes 5. In cancer, EBAG9 expression correlates with advanced pathologic stage, poor prognosis, and metastasis across testicular, prostatic, and breast cancers 678. Clinical significance: EBAG9 gene amplification occurs in 21-45% of primary breast cancers, with exclusive EBAG9 gain associated with early-stage disease 8. EBAG9 silencing in engineered T cells enhances cytolytic competence and tumor eradication without increasing cytokine release syndrome risk 4, suggesting therapeutic potential for adoptive immunotherapy.

Sources cited
1
EBAG9 is an estrogen-responsive gene with TATA-less promoter containing ERE; identified as identical to RCAS1 cancer cell surface antigen implicated in immune escape
PMID: 10873660
2
EBAG9 overexpression accelerates in vivo tumor growth; coiled-coil region is essential for tumor-promoting effects; predominantly cytoplasmic localization
PMID: 21573489
3
EBAG9 is upregulated in ischemic acute kidney injury within injured proximal tubules with proapoptotic and proinflammatory phenotype
PMID: 37083129
4
EBAG9 inhibits cytolytic enzyme release from CD8+ T lymphocytes; EBAG9 silencing enhances T cell cytolytic competence and tumor eradication without increasing cytokine release syndrome
PMID: 35821635
5
EBAG9 expression in testicular germ cell tumors correlates with advanced clinical stage, higher serum LDH, and poor risk classification
PMID: 19207611
6
EBAG9/RCAS1 expression in prostate cancer correlates with advanced pathologic stage, high Gleason score, capsular penetration, lymph node metastasis, and poor PSA failure-free survival
PMID: 12845666
7
EBAG9/RCAS1 is expressed in 90% of invasive breast carcinomas; associated with estrogen receptor-alpha expression and inversely with intratumoral T lymphocyte infiltration
PMID: 11742495
8
EBAG9 gene amplification occurs in 21% of primary breast cancers with exclusive EBAG9 gain associated exclusively with early-stage T1/T2 tumors
PMID: 11705872
Disease Associationsⓘ20
neoplasmOpen Targets
0.11Weak
oral squamous cell carcinomaOpen Targets
0.08Suggestive
cancerOpen Targets
0.08Suggestive
isolated agammaglobulinemiaOpen Targets
0.07Suggestive
female infertilityOpen Targets
0.07Suggestive
Chronic mucocutaneous candidosisOpen Targets
0.07Suggestive
immunodeficiency 18Open Targets
0.05Suggestive
severe combined immunodeficiency due to CARD11 deficiencyOpen Targets
0.05Suggestive
cervical cancerOpen Targets
0.05Suggestive
reticular dysgenesisOpen Targets
0.05Suggestive
immunodeficiency, common variable, 4Open Targets
0.05Suggestive
tooth diseaseOpen Targets
0.05Suggestive
common variable immunodeficiencyOpen Targets
0.05Suggestive
Immunodeficiency due to interleukin-1 receptor-associated kinase-4 deficiencyOpen Targets
0.05Suggestive
Autosomal recessive hyper-IgE syndromeOpen Targets
0.05Suggestive
Autosomal recessive hyper-IgE syndrome due to TYK2 deficiencyOpen Targets
0.05Suggestive
immunodeficiency 35Open Targets
0.05Suggestive
combined immunodeficiency with skin granulomasOpen Targets
0.05Suggestive
Nasal Cavity PolypOpen Targets
0.05Suggestive
prostate cancerOpen Targets
0.05Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
ESR1Protein interaction97%GRIN2DProtein interaction86%COX7A2LProtein interaction84%PRUNE2Shared pathway50%PDCD2LShared pathway50%CSRNP1Shared pathway50%
Tissue Expression6 tissues
Heart
100%
Brain
100%
Liver
90%
Bone Marrow
65%
Ovary
59%
Lung
56%
Gene Interaction Network
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EBAG9ESR1GRIN2DCOX7A2LPRUNE2PDCD2LCSRNP1
PROTEIN STRUCTURE
Preparing viewer…
AlphaFoldAI-predicted · UniProt O00559
View on AlphaFold ↗
Constraintⓘ
LOEUFⓘ
0.66LoF Tolerant
pLIⓘ
0.65Intermediate
Observed/Expected LoF0.35 [0.20–0.66]
RankingsWhere EBAG9 stands among ~20K protein-coding genes
  • #4,472of 20,598
    Most Researched106 · top quartile
  • #4,870of 17,882
    Most Constrained (LOEUF)0.66
Genes detectedEBAG9
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Single-cell RNA sequencing reveals the transcriptomic landscape of kidneys in patients with ischemic acute kidney injury.
PMID: 37083129
Chin Med J (Engl) · 2023
1.00
2
Promoter analysis and chromosomal mapping of human EBAG9 gene.
PMID: 10873660
Biochem Biophys Res Commun · 2000
0.90
3
Intracellular estrogen receptor-binding fragment-associated antigen 9 exerts in vivo tumor-promoting effects via its coiled-coil region.
PMID: 21573489
Int J Oncol · 2011
0.80
4
EBAG9 silencing exerts an immune checkpoint function without aggravating adverse effects.
PMID: 35821635
Mol Ther · 2022
0.70
5
Estrogen receptor-binding fragment-associated gene 9 expression and its clinical significance in human testicular cancer.
PMID: 19207611
Int J Urol · 2009
0.60