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10 sources retrieved · Most recent: April 2026 · Index updated 14 days ago
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EML4
EMAP like 4
Chromosome 2 · 2p21
NCBI Gene: 27436Ensembl: ENSG00000143924.19HGNC: HGNC:1316UniProt: B5MBZ0
146PubMed Papers
20Diseases
6Drugs
0Pathogenic Variants
CLINICAL
FDA Approved Target
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
protein bindingcytosolmicrotubulemicrotubule cytoskeletonnon-small cell lung carcinomacancerAbnormality of the skeletal systemneoplasm
✦AI Summary

EML4 (EMAP like 4) is a microtubule-associated protein essential for microtubule formation and stability 12. It plays a critical role in organizing the mitotic spindle and mediating kinetochore-microtubule attachment during cell division 3. Beyond its canonical mitotic functions, EML4 has emerged as a novel metastatic driver in lung adenocarcinoma through two distinct mechanisms. First, EML4 can form transforming fusion proteins with anaplastic lymphoma kinase (ALK) through chr2 inversions, occurring in approximately 6.7% of non-small-cell lung cancer (NSCLC) patients 4. EML4-ALK variants with different EML4 breakpoints display distinct biological and clinical properties, with variant 3 conferring worse prognosis and higher metastatic risk compared to variant 1 56. Second, wild-type EML4 is hypermethylated in primary lung tumors, promoting translation and overexpression, which enhances cytoskeleton dynamics, lamellipodia formation, cellular motility, and metastasis through interaction with ARPC1A 7. Clinically, ALK inhibitors are standard therapy for EML4-ALK-positive NSCLC, though resistance emerges through kinase domain mutations like G1202R 58. Understanding EML4-ALK variant-specific biology and wild-type EML4's metastatic role offers opportunities for optimized therapeutic strategies.

Sources cited
1
EML4 is essential for microtubule formation and stability
PMID: 16890222
2
EML4 is required for microtubule formation and stability
PMID: 31409757
3
EML4 is required for mitotic spindle organization and kinetochore-microtubule attachment
PMID: 25789526
4
EML4-ALK fusion gene occurs in 6.7% of NSCLC patients and is transforming
PMID: 17625570
5
EML4-ALK variants have distinct properties; variant 3 worsens prognosis and increases metastatic risk; G1202R mutation causes ALK inhibitor resistance
PMID: 37149843
6
EML4-ALK variant 3 is more oncogenic than variant 1; variants have variant-specific effects on tumorigenesis
PMID: 40986428
7
Wild-type EML4 is hypermethylated in lung adenocarcinoma, promotes translation and overexpression, enhances cytoskeleton dynamics and metastasis through ARPC1A interaction
PMID: 38922581
8
ALK inhibitors are effective first-line and further-line treatments for EML4-ALK-positive NSCLC
PMID: 39796163
9
EML4-ALK variants do not significantly affect PFS or treatment response to ALK inhibitors like alectinib
PMID: 30902613
Disease Associationsⓘ20
non-small cell lung carcinomaOpen Targets
0.70Strong
cancerOpen Targets
0.65Moderate
Abnormality of the skeletal systemOpen Targets
0.63Moderate
neoplasmOpen Targets
0.62Moderate
lung cancerOpen Targets
0.51Moderate
lung adenocarcinomaOpen Targets
0.40Weak
papillary thyroid carcinomaOpen Targets
0.37Weak
lung carcinomaOpen Targets
0.37Weak
squamous cell lung carcinomaOpen Targets
0.37Weak
Bladder AdenocarcinomaOpen Targets
0.37Weak
lymphomaOpen Targets
0.37Weak
large cell lung carcinomaOpen Targets
0.37Weak
acinar lung adenocarcinomaOpen Targets
0.37Weak
adenosquamous lung carcinomaOpen Targets
0.37Weak
bronchoalveolar adenocarcinomaOpen Targets
0.37Weak
carcinoma of liver and intrahepatic biliary tractOpen Targets
0.37Weak
follicular thyroid carcinomaOpen Targets
0.37Weak
Poorly Differentiated Thyroid Gland CarcinomaOpen Targets
0.37Weak
cervical carcinomaOpen Targets
0.33Weak
infantile hypertrophic pyloric stenosisOpen Targets
0.32Weak
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Drug Targets6
ALECTINIBApproved
EML4-ALK inhibitor
lung cancer
ALECTINIB HYDROCHLORIDEApproved
EML4-ALK inhibitor
non-small cell lung carcinoma
CERITINIBApproved
NPM/ALK (Nucleophosmin/ALK tyrosine kinase receptor) inhibitor
CRIZOTINIBApproved
EML4-ALK inhibitor
non-small cell lung carcinoma
LORLATINIBApproved
ALK tyrosine kinase receptor inhibitor
lymphoma
PLB1003Phase I
ALK tyrosine kinase receptor inhibitor
non-small cell lung carcinoma
Related Genes
PIK3R3Protein interaction100%PIK3R1Protein interaction100%PIK3CBProtein interaction100%PIK3CDProtein interaction100%NEK7Protein interaction100%BRAFProtein interaction100%
Tissue Expression6 tissues
Bone Marrow
100%
Liver
69%
Lung
59%
Ovary
30%
Brain
20%
Heart
15%
Gene Interaction Network
Click a node to explore
EML4PIK3R3PIK3R1PIK3CBPIK3CDNEK7BRAF
PROTEIN STRUCTURE
Preparing viewer…
PDB4CGC · 2.90 Å · X-ray
View on RCSB ↗
Constraintⓘ
LOEUFⓘ
0.68LoF Tolerant
pLIⓘ
0.00Tolerant
Observed/Expected LoF0.53 [0.42–0.68]
RankingsWhere EML4 stands among ~20K protein-coding genes
  • #3,102of 20,598
    Most Researched146 · top quartile
  • #294of 1,025
    FDA-Approved Drug Targets5
  • #5,059of 17,882
    Most Constrained (LOEUF)0.68
Genes detectedEML4
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer.
PMID: 17625570
Nature · 2007
1.00
2
EML4-ALK: Update on ALK Inhibitors.
PMID: 39796163
Int J Mol Sci · 2025
0.90
3
The N6-methyladenosine Epitranscriptomic Landscape of Lung Adenocarcinoma.
PMID: 38922581
Cancer Discov · 2024
0.80
4
EML4-ALK biology and drug resistance in non-small cell lung cancer: a new phase of discoveries.
PMID: 37149843
Mol Oncol · 2023
0.70
5
EML4-ALK Variant-Specific Genetic Interactions Shape Lung Tumorigenesis.
PMID: 40986428
Cancer Discov · 2026
0.60