GNL2 (G protein nucleolar 2) is a GTPase that plays a critical role in ribosome biogenesis and cell proliferation. The protein associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation 1. GNL2 influences 60S ribosomal subunit maturation and global protein synthesis, providing a mechanism for its oncogenic effects 1. The protein serves as a core component of the RNAmetasome network involved in macromolecule biogenesis, where it interacts with other key proteins like MKI67 and MDN1 2. GNL2 knockdown significantly retards cell proliferation and affects the state of network proteins 2. In cancer contexts, GNL2 is frequently upregulated and associated with poor prognosis. Overexpression stimulates proliferation and colony formation, while silencing impairs cancer cell growth and diminishes xenograft tumor formation 1. GNL2 has been identified as a ribosomal maturation factor and downstream regulator in ribosome biogenesis pathways 3. The protein has been implicated in various cancers including ovarian cancer, hepatocellular carcinoma, and lung adenocarcinoma, making it a potential therapeutic target 143.