GTF2H2C is a component of the core-TFIIH basal transcription factor complex, participating in nucleotide excision repair (NER) of DNA and RNA polymerase II-mediated transcription when complexed with CAK. Beyond its canonical transcriptional roles, GTF2H2C shows clinical relevance in developmental and neoplastic contexts. In utero nicotine exposure is associated with differential DNA methylation at GTF2H2C loci in placental tissue, suggesting involvement in the fetal origins of chr5 diseases including asthma and immune disorders 1. GTF2H2C has been identified as a putative tumor antigen in gliomas, with potential utility for mRNA vaccine development targeting immune-favorable glioma subtypes 2. Additionally, GTF2H2C functions as a prognostic enhancer RNA in non-small cell lung cancer (NSCLC) metastasis, demonstrating predictive value for metastatic disease progression 3. These findings indicate that beyond its established function in transcription and DNA repair, GTF2H2C represents a multi-dimensional biomarker relevant to environmental exposure effects, immunotherapy development, and cancer prognosis.