GTPBP4 (GTP binding protein 4) is a multifunctional protein that plays crucial roles in ribosomal biogenesis and oncogenesis. The protein is involved in the biogenesis of the 60S ribosomal subunit 1 and exhibits GTPase activity essential for cellular processes. GTPBP4 functions as a negative regulator of the p53 tumor suppressor pathway by directly interacting with p53, preventing its stabilization and subsequent cell cycle arrest 2. In cancer contexts, GTPBP4 is consistently overexpressed across multiple tumor types including hepatocellular carcinoma, gastric cancer, non-small cell lung cancer, and colorectal cancer 3245. The protein promotes tumor progression through several mechanisms: it enhances aerobic glycolysis by inducing PKM2 sumoylation and dimerization 6, facilitates epithelial-mesenchymal transition (EMT) 4, and negatively regulates innate immune responses by inhibiting IRF3 binding to interferon promoters 7. Additionally, GTPBP4 enhances oocyte developmental competence by promoting cell cycle progression and organelle maturation 8. High GTPBP4 expression correlates with poor prognosis in multiple cancer types, making it a potential therapeutic target and prognostic biomarker 35.