GXYLT2 (glucoside xylosyltransferase 2) is a glycosyltransferase that modifies Notch signaling through O-linked glycosylation. The enzyme catalyzes the addition of xylose to O-glucose residues on epidermal growth factor (EGF)-like repeats of Notch receptors 1. GXYLT2 functions as a positive regulator of Notch pathway activation, promoting cell proliferation and migration while activating MAPK phosphorylation 1. The protein is significantly overexpressed across multiple cancer types, where it correlates with poor prognosis and advanced disease characteristics 23. In gastric cancer, elevated GXYLT2 expression associates with tumor grade, stage, and invasion depth, serving as an independent prognostic factor 2. The gene also shows clinical relevance in bladder cancer 4, clear cell renal cell carcinoma where it's regulated by microRNA-204-5p 5, and heart failure where it contributes to pathological cardiac remodeling 6. GXYLT2 expression correlates with immune cell infiltration patterns in tumors, suggesting roles in tumor immunity 24. Additionally, GXYLT2 has been identified as relevant to ulcerative colitis through O-glycosylation mechanisms 7. These findings establish GXYLT2 as a potential therapeutic target and biomarker across multiple diseases.