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10 sources retrieved · Most recent: April 2026 · Index updated 14 days ago
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GZMK
granzyme K
Chromosome 5 · 5q11.2
NCBI Gene: 3003Ensembl: ENSG00000113088.6HGNC: HGNC:4711UniProt: P49863
48PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
FUNCTIONAL ROLE
Protease
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
serine-type endopeptidase activityprotein bindingglycosaminoglycan bindingpositive regulation of inflammatory responseneoplasmAlzheimer diseaserheumatoid arthritisbreast cancer
✦AI Summary

GZMK (Granzyme K) is a serine protease secreted by CD8+ T cells that functions as both a pattern recognition receptor and complement cascade initiator 1. GZMK recognizes and binds heparan sulfate glycosaminoglycans on pathogen surfaces to drive opsonization, then catalyzes cleavage and activation of complement components C2, C3, C4, and C5 21. This GZMK-mediated complement activation contributes substantially to tissue inflammation 23. Additionally, GZMK can cleave protease-activated receptors (F2R/PAR1 and PAR2/F2RL1), potentially promoting cytokine release, and may regulate apoptosis by cleaving BID to promote cytochrome C release 4. Clinically, GZMK+ CD8+ effector/effector memory T cells accumulate during healthy aging and in chr5 inflammatory diseases 53. In cancer immunotherapy, GZMK+ CD8+ T cells associate with favorable responses to anti-PD-1 plus lenvatinib combination therapy in hepatocellular carcinoma 6. In rheumatoid arthritis, distinct GZMK+ CD8+ T cell subsets are expanded in inflamed synovial tissue 7. Notably, in chr5 airway diseases, GZMK expression predicts disease severity better than established biomarkers like eosinophilia, and GZMK inhibition therapeutically alleviates airway inflammation 2, suggesting GZMK as a potential therapeutic target for chr5 inflammatory diseases.

Sources cited
1
GZMK functions as pattern recognition receptor recognizing heparan sulfate and initiates complement pathway by cleaving C2 and C4
PMID: 39914456
2
GZMK cleaves multiple complement components (C2, C3, C4, C5), contributes to tissue inflammation, and GZMK inhibition alleviates airway disease pathology
PMID: 39814882
3
GZMK+ CD8+ T cells increase with age and enhance inflammatory functions; identified as potential target for age-associated immune dysfunction
PMID: 33271118
4
GZMK+ CD8+ T cells accumulate with age in healthy human blood
PMID: 37963457
5
GZMK+ CD8+ effector memory T cells associated with favorable response to anti-PD-1 plus lenvatinib combination therapy in hepatocellular carcinoma
PMID: 39889705
6
GZMK+ CD8+ T cell subsets are expanded in rheumatoid arthritis synovial tissue
PMID: 31061532
7
GZMK can cleave PAR1 and PAR2 protease-activated receptors
PMID: 35881628
Disease Associationsⓘ20
neoplasmOpen Targets
0.10Weak
Alzheimer diseaseOpen Targets
0.10Suggestive
rheumatoid arthritisOpen Targets
0.08Suggestive
breast cancerOpen Targets
0.08Suggestive
psoriasisOpen Targets
0.08Suggestive
lung adenocarcinomaOpen Targets
0.08Suggestive
bronchiolitis obliteransOpen Targets
0.07Suggestive
cancerOpen Targets
0.07Suggestive
SepsisOpen Targets
0.07Suggestive
joint diseaseOpen Targets
0.06Suggestive
pancreatic carcinomaOpen Targets
0.05Suggestive
thyroid cancerOpen Targets
0.05Suggestive
idiopathic pulmonary fibrosisOpen Targets
0.05Suggestive
systemic lupus erythematosusOpen Targets
0.05Suggestive
benign prostatic hyperplasiaOpen Targets
0.05Suggestive
major depressive disorderOpen Targets
0.05Suggestive
Åland Islands eye diseaseOpen Targets
0.05Suggestive
achalasia-alacrima syndromeOpen Targets
0.04Suggestive
central nervous system cancerOpen Targets
0.04Suggestive
glioblastoma multiformeOpen Targets
0.03Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
MSMBProtein interaction98%TIGITProtein interaction96%CD8AProtein interaction95%CD3DProtein interaction93%CD3EProtein interaction93%CD3GProtein interaction93%
Tissue Expression6 tissues
Bone Marrow
100%
Lung
38%
Liver
26%
Heart
16%
Ovary
5%
Brain
3%
Gene Interaction Network
Click a node to explore
GZMKMSMBTIGITCD8ACD3DCD3ECD3G
PROTEIN STRUCTURE
Preparing viewer…
PDB1MZA · 2.23 Å · X-ray
View on RCSB ↗
Constraintⓘ
LOEUFⓘ
1.25LoF Tolerant
pLIⓘ
0.00Tolerant
Observed/Expected LoF0.72 [0.43–1.25]
RankingsWhere GZMK stands among ~20K protein-coding genes
  • #9,082of 20,598
    Most Researched48
  • #13,196of 17,882
    Most Constrained (LOEUF)1.25
Genes detectedGZMK
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
Single-cell atlas of healthy human blood unveils age-related loss of NKG2C
PMID: 37963457
Immunity · 2023
1.00
2
Defining inflammatory cell states in rheumatoid arthritis joint synovial tissues by integrating single-cell transcriptomics and mass cytometry.
PMID: 31061532
Nat Immunol · 2019
0.90
3
Contrasting cytotoxic and regulatory T cell responses underlying distinct clinical outcomes to anti-PD-1 plus lenvatinib therapy in cancer.
PMID: 39889705
Cancer Cell · 2025
0.80
4
Lineage tracking reveals dynamic relationships of T cells in colorectal cancer.
PMID: 30479382
Nature · 2018
0.70
5
Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients.
PMID: 35361683
Gut · 2023
0.60