JPT1 (Jupiter microtubule associated homolog 1), also known as HN1, is a multifunctional protein involved in cell signaling, cell cycle regulation, and cancer progression. Functionally, JPT1 negatively modulates AKT-mediated GSK3B signaling, inducing β-catenin (CTNNB1) phosphorylation and degradation through suppression of inhibitory GSK3B phosphorylation, thereby repressing adherens junction function and E-cadherin interaction 1. JPT1 plays regulatory roles in cell cycle and cell adhesion 12. Additionally, JPT1 inhibits androgen receptor signaling through proteasomal degradation 3. At the cellular level, JPT1 interacts with γ-tubulin to regulate centrosome function and microtubule spindle assembly in prostate cancer cells 4. In cancer pathology, JPT1 is significantly upregulated across multiple malignancies including renal cell carcinoma, prostate cancer, and endometrial cancer, correlating with aggressive phenotypes and poor prognosis 56. JPT1 elevation promotes cancer cell proliferation, migration, invasion, and glycolytic metabolism through the circRNA/miRNA/JPT1 regulatory axes 57. JPT1 serves as both a predictive and pharmacodynamic biomarker of metformin response in endometrial cancer 8. These findings establish JPT1 as a significant oncogenic driver and potential therapeutic target in cancer management.