KLK14 (kallikrein related peptidase 14) is a serine protease with dual trypsin-like and chymotrypsin-like substrate specificity that plays important roles in normal physiology and cancer progression 1. The enzyme is primarily expressed in the central nervous system, prostate, and skeletal muscle, with highest levels found in brain, cerebellum, and spinal cord 21. KLK14 functions in epidermal desquamation through desmoglein DSG1 cleavage and can activate other kallikreins including KLK1, KLK3, KLK5, and KLK11 3. In reproductive physiology, it may contribute to seminal clot liquefaction through direct cleavage of semeno-gelins. Dysregulated KLK14 expression is associated with multiple cancers, showing significant overexpression in prostate cancer tissues compared to normal counterparts, with higher levels correlating with advanced stage and grade 4. Similarly, KLK14 is overexpressed in breast cancer and associated with higher tumor grades and positive nodal status 56. In cervical cancer, KLK14 promotes tumorigenesis through PAR-2-dependent RhoA and NF-κB signaling pathways 7. The protein appears to function as a pro-tumorigenic factor involved in growth, invasion, and angiogenesis, making it a potential diagnostic and therapeutic target for multiple cancer types.