MT4-MMP (also known as MMP-17) is a glycosylphosphatidylinositol (GPI)-anchored membrane-type matrix metalloproteinase that plays distinct roles in extracellular matrix remodeling and cancer progression 1. Unlike other MT-MMPs, MT4-MMP is uniquely anchored to the plasma membrane via a GPI motif, conferring distinct regulatory and functional mechanisms 2. The protein undergoes complex processing, with three molecular forms (45 kDa, 58 kDa mature form, and 69 kDa precursor) identified in melanoma cells, where the precursor is processed by furin cleavage in the Golgi apparatus 3. MT4-MMP promotes cancer cell migration and invasion through multiple mechanisms, including stimulation of invadopodia formation via interaction with Tks5 and PDGFRα leading to Src activation, and enhancement of amoeboid-like cell movement through activation of Rho and Cdc42 GTPases 4. The enzyme is highly expressed across various cancer types and contributes to tumor progression, metastasis, and drug resistance 56. MT4-MMP represents a potential therapeutic target and biomarker for cancer diagnosis and monitoring due to its involvement in multiple pathological processes 7.