NF1 (neurofibromin 1) is a tumor suppressor gene located on chromosome 17.2 that encodes neurofibromin, a critical regulator of cellular growth and proliferation. The primary function of neurofibromin is to stimulate GTPase activity of the proto-oncogene Ras, thereby downregulating the RAS/MAPK signaling pathway 12. Additionally, neurofibromin controls cell growth through regulation of cyclic adenosine monophosphate (AMP) 1. Beyond Ras regulation, NF1 loss impairs Schwann cell differentiation by inducing a persistent stem-like state that expands progenitor pools 3. Constitutional NF1 mutations cause neurofibromatosis type 1 (NF1), an autosomal dominant neurocutaneous disorder characterized by benign peripheral nerve sheath tumors (neurofibromas), café-au-lait macules, and predisposition to malignancies including breast cancer and leukemia 14. Somatic NF1 mutations also occur frequently in sporadic cancers (melanoma, lung, colorectal, ovarian, glioblastoma), indicating broader roles in cancer pathogenesis 2. NF1 mutations contribute to drug resistance in melanoma, lung, and breast cancers 2. Comprehensive RNA analysis achieves ~96-97% diagnostic sensitivity in classically affected NF1 patients 5, and recent therapeutic approaches including econazole have shown promise in preclinical models of cutaneous neurofibromas 6.