NME7 is a multifunctional protein with distinct kinase and nuclease activities that plays critical roles in cellular signaling and ciliary function. The protein possesses intrinsic kinase activity that phosphorylates GSK3B at serine-9, leading to activation of Wnt/β-catenin signaling, which promotes one-carbon metabolism in hepatocellular carcinoma 1. NME7 also exhibits 3'-5'-DNA exonuclease activity, removing single nucleotides from DNA substrates and participating in DNA nucleolytic processing 2. As a functional component of the gamma-tubulin ring complex, NME7 regulates microtubule-nucleating activity at centrosomes in a kinase-dependent manner and maintains primary cilium assembly and ciliary microtubule stability 3. The protein localizes to centrosomes and basal bodies, with deficiency impacting Hedgehog signaling through reduced smoothened localization within primary cilia 3. Clinically, NME7 upregulation correlates with poor prognosis in hepatocellular carcinoma 1, while genetic variants associate with glucose metabolism and insulin sensitivity 4. NME7 knockout mice exhibit ciliary dyskinesia-like lesions, confirming its essential role in ciliary function 5.