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10 sources retrieved · Most recent: April 2026 · Index updated 14 days ago
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NSFL1C
NSFL1 cofactor
Chromosome 20 · 20p13
NCBI Gene: 55968Ensembl: ENSG00000088833.19HGNC: HGNC:15912UniProt: Q53FE8
135PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
negative regulation of protein localization to centrosomeestablishment of mitotic spindle orientationpositive regulation of mitotic centrosome separationprotein bindingneurodegenerative diseaseadult T-cell leukemia/lymphomaplacenta praevianon-alcoholic fatty liver disease
✦AI Summary

NSFL1C (NSFL1 cofactor, also known as p47) is a VCP cofactor that regulates multiple cellular processes critical for mitotic progression and neurodegeneration. Functionally, NSFL1C reduces VCP ATPase activity and is necessary for Golgi stack fragmentation during mitosis and reassembly afterward 1. It regulates centrosomal Aurora A (AURKA) levels during mitosis by promoting removal from centrosomes in prophase and controls spindle orientation 1. NSFL1C functions within a PINK1-VCP-PKA signaling pathway where PINK1 phosphorylates p47 at S176 to promote dendritic arborization and spinogenesis in neurons 23. In tau seeding pathways relevant to tauopathies, NSFL1C reduction decreases tau seed amplification within 5 hours of exposure, suggesting VCP-NSFL1C complexes direct pathological tau toward degradation rather than replication 45. NSFL1C also regulates autophagy flux and lysosomal homeostasis; p47 ablation increases HER2+ breast cancer metastasis by impairing lysosomal damage repair and NEMO trafficking 6. Clinically, reduced NSFL1C expression correlates with increased metastasis and decreased survival in breast cancer patients 6. Additionally, NSFL1C loss suppresses spindle assembly checkpoint activation in BRCA2-deficient prostate cancer, promoting cell survival 7.

Sources cited
1
NSFL1C regulates centrosomal AURKA levels and spindle orientation during mitosis
PMID: 23649807
2
PINK1 phosphorylates NSFL1C/p47 at S176 to promote dendritic complexity through PKA activation
PMID: 30783609
3
PINK1-regulated p47 phosphorylation is required for dendritic branching and spine development
PMID: 36414008
4
NSFL1C reduction decreases tau seed amplification within 5 hours, indicating VCP-NSFL1C directs tau toward degradation
PMID: 39773263
5
NSFL1C reduction decreases tau seeding in VCP-dependent cytoplasmic processing complex
PMID: 38826306
6
NSFL1C/p47 suppresses HER2+ breast cancer metastasis by regulating lysosomal homeostasis and NEMO trafficking; low p47 correlates with metastasis and poor survival
PMID: 38363674
7
NSFL1C loss suppresses spindle assembly checkpoint activation in BRCA2-deficient prostate cancer, promoting cell survival
PMID: 37934606
Disease Associationsⓘ20
neurodegenerative diseaseOpen Targets
0.33Weak
adult T-cell leukemia/lymphomaOpen Targets
0.07Suggestive
placenta praeviaOpen Targets
0.05Suggestive
non-alcoholic fatty liver diseaseOpen Targets
0.02Suggestive
breast cancerOpen Targets
0.02Suggestive
acute lymphoblastic leukemiaOpen Targets
0.01Suggestive
idiopathic pulmonary fibrosisOpen Targets
0.01Suggestive
bacteriemiaOpen Targets
0.01Suggestive
chronic granulomatous diseaseOpen Targets
0.01Suggestive
meningitisOpen Targets
0.01Suggestive
Hyperplastic PolypOpen Targets
0.01Suggestive
neoplasmOpen Targets
0.01Suggestive
infectionOpen Targets
0.01Suggestive
ImmunodeficiencyOpen Targets
0.01Suggestive
Insulin resistanceOpen Targets
0.01Suggestive
obesityOpen Targets
0.01Suggestive
SepsisOpen Targets
0.01Suggestive
prostate cancerOpen Targets
0.01Suggestive
experimental autoimmune encephalomyelitisOpen Targets
0.01Suggestive
T-cell leukemiaOpen Targets
0.01Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
UBXN8Protein interaction100%UBE4AProtein interaction100%PLAAProtein interaction100%UBE4BProtein interaction100%FAF1Protein interaction100%UBXN7Protein interaction100%
Tissue Expression6 tissues
Brain
100%
Heart
100%
Lung
94%
Bone Marrow
94%
Liver
90%
Ovary
88%
Gene Interaction Network
Click a node to explore
NSFL1CUBXN8UBE4APLAAUBE4BFAF1UBXN7
PROTEIN STRUCTURE
Preparing viewer…
PDB8HRZ · 2.70 Å · X-ray
View on RCSB ↗
Constraintⓘ
LOEUFⓘ
0.55Moderately Constrained
pLIⓘ
0.92Intolerant
Observed/Expected LoF0.33 [0.20–0.55]
RankingsWhere NSFL1C stands among ~20K protein-coding genes
  • #3,431of 20,598
    Most Researched135 · top quartile
  • #3,546of 17,882
    Most Constrained (LOEUF)0.55 · top quartile
Genes detectedNSFL1C
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
PP2A inhibition causes synthetic lethality in BRCA2-mutated prostate cancer models via spindle assembly checkpoint reactivation.
PMID: 37934606
J Clin Invest · 2024
1.00
2
VCP regulates early tau seed amplification via specific cofactors.
PMID: 39773263
Mol Neurodegener · 2025
0.90
3
PINK1 Interacts with VCP/p97 and Activates PKA to Promote NSFL1C/p47 Phosphorylation and Dendritic Arborization in Neurons.
PMID: 30783609
eNeuro · 2018
0.80
4
VCP regulates early tau seed amplification via specific cofactors.
PMID: 38826306
Res Sq · 2024
0.70
5
Differential expression of novel tyrosine kinase substrates during breast cancer development.
PMID: 17855441
Mol Cell Proteomics · 2007
0.60