NUDT6 (nudix hydrolase 6) is a mitochondrial enzyme that mediates the hydrolysis of nucleoside diphosphate derivatives, particularly NADH and FAD 1. The enzyme localizes exclusively to mitochondria and exhibits distinct substrate specificity, being mainly active towards NADH compared to other NUDIX family members 1. NUDT6 depletion results in increased respiratory chain activity and altered mitochondrial respiratory chain complex expression, suggesting a regulatory role in mitochondrial metabolism 1. Beyond its enzymatic function, NUDT6 serves as an antisense transcript that negatively regulates fibroblast growth factor 2 (FGF2) expression through transcriptional mechanisms 2. This antisense regulation is mediated by chr4-remodeling factors and histone modifications, creating a strong negative correlation between NUDT6 and FGF2 levels 2. NUDT6 contributes to negative regulation of cell proliferation, with overexpression impairing smooth muscle cell migration while limiting proliferation and augmenting apoptosis 3. The gene shows clinical relevance in various diseases, including associations with fat deposition traits 4, differential expression across colorectal cancer stages 5, and potential as an immune biomarker in coronary heart disease and osteosarcoma 67. Green tea catechins can suppress NUDT6 expression post-transcriptionally, potentially contributing to anti-proliferative effects 8.