PFKFB1 encodes a bifunctional enzyme that catalyzes both the synthesis and degradation of fructose-2,6-bisphosphate (F-2,6-BP), a critical regulator of glycolysis 12. This enzyme controls glycolytic flux by modulating the steady-state concentration of F-2,6-BP, which allosterically activates 6-phosphofructo-1-kinase, a rate-limiting enzyme in glycolysis 2. The gene is located on chromosome X at position Xp11.21, approximately 250 kb telomeric to the ALAS2 gene 3. PFKFB1 expression is transcriptionally regulated by SREBP-1a through a sterol regulatory element in its promoter, mediating postprandial activation in response to glucose and insulin 1. Multiple mRNA variants exist, including a novel fetal liver-specific isoform that may facilitate the metabolic transition from fetal to adult liver metabolism 4. In disease contexts, PFKFB1 has been implicated in multiple myeloma progression through the STAT3-PFKFB1 pathway 5. While PFKFB family members are considered potential therapeutic targets for cancer treatment due to their role in tumor cell glycolysis 67, PFKFB1's specific role in cancer metabolism requires further investigation.