PIP5KL1 (phosphatidylinositol-4-phosphate 5-kinase like 1) functions as a scaffolding protein that localizes and regulates type I PI(4)P 5-kinases to generate phosphatidylinositol 4,5-bisphosphate (PIP2) at specific cellular compartments, supporting actin dynamics, signal transduction, and protein recruitment 1. The protein negatively regulates cell proliferation and migration in cancer contexts. Overexpression of PIP5KL1 significantly suppressed cervical cancer HeLa cell growth both in vitro and in vivo, with reduced cell proliferation and migration 2. Similarly, PIP5KL1 overexpression in gastric cancer cells (BGC823) inhibited proliferation and migration while suppressing AKT1 phosphorylation, suggesting inhibition of PI3K/AKT signaling 3. PIP5KL1 was identified in cell-based screening as capable of inducing both necrosis and apoptosis, indicating roles in cell viability regulation 4. In canine prostate cancer, PIP5KL1 expression was upregulated alongside other PI3K/AKT/mTOR pathway components in tumor cells compared to normal tissue 5, suggesting context-dependent dysregulation in malignancy. These findings indicate PIP5KL1 functions as a tumor suppressor, with potential therapeutic implications for cancers exhibiting elevated PI3K/AKT signaling.