PLB1 encodes phospholipase B1, a calcium-independent membrane-associated enzyme that catalyzes the complete hydrolysis of phospholipids by cleaving both sn-1 and sn-2 fatty acyl chains from the glycerol backbone. The enzyme exhibits dual phospholipase and lysophospholipase activities with preferential cleavage of sn-2 ester bonds and can also act as a lipase toward glycerolipids and retinyl esters. In fungal pathogens, PLB1 plays critical roles in virulence and pathogenesis. In Candida albicans, PLB1 expression is regulated during morphogenic transitions and upregulated in biofilm formation on medical devices in the presence of human serum 12. Reintroduction studies demonstrate that PLB1 enhances the ability of C. albicans to cross gastrointestinal mucosa and cause systemic infection 3. In Cryptococcus neoformans, PLB1 is essential for maintaining capsular elasticity, regulating polysaccharide secretion, and biofilm formation critical for colonization 4. In fission yeast, PLB1 mutations can extend chr2 lifespan by activating stress response pathways 5. Additionally, PLB1 has been identified as a potential biomarker, with reduced plasma levels inversely associated with peripheral artery disease risk in type 2 diabetes patients 6 and implicated in intervertebral disc degeneration through arachidonic acid metabolism 7.