PRKD1 encodes protein kinase D1, a serine/threonine kinase that serves as a critical signaling hub in multiple cellular processes. The protein functions downstream of protein kinase C (PKC) to transduce diacylglycerol signals, regulating diverse pathways including NF-κB activation, MAPK signaling, and Golgi membrane trafficking 1. PRKD1 plays essential roles in maintaining endothelial barrier integrity through phosphorylation of α-catenin at Thr654, which regulates adherens junction stability and vascular permeability 2. In cancer biology, PRKD1 is a direct transcriptional target of Twist1 and is required for epithelial dissemination and metastasis in breast cancer models 3. The gene is also targeted by oncogenic KRas-NF-κB signaling in pancreatic cancer, linking it to oncogenic processes 1. PRKD1 expression is regulated by transcription factors Sp1, Sp3, and NF-κB-p65, with epigenetic mechanisms including DNA methylation and histone modifications controlling its expression 4. Clinically, PRKD1 mutations cause telangiectasia-ectodermal dysplasia-brachydactyly-cardiac anomaly (TEBC) syndrome, characterized by congenital heart defects, ectodermal dysplasia, and neurodevelopmental disorders 56. The protein also shows protective functions in dopaminergic neurons against oxidative stress, suggesting roles in neurodegeneration 4.