PRKRIP1 (PRKR interacting protein 1) is a splicing factor essential for pre-mRNA splicing. Structurally, PRKRIP1 forms a 100-Å α-helix within the human spliceosome that links the U2 snRNP to the catalytic center 1. During the C-to-C* transition, PRKRIP1 is recruited as a step II factor alongside other splicing components to facilitate exon ligation 2. Additionally, PRKRIP1 exhibits double-stranded RNA binding capacity and inhibits protein kinase R (PKR) activation through a dsRNA-independent mechanism 3. Clinically, PRKRIP1 has emerged as a significant prognostic biomarker in colorectal cancer (CRC). High PRKRIP1 expression is associated with poor prognosis, with elevated levels correlating with apoptosis-related gene sets 4. A four-gene prognostic model incorporating PRKRIP1 accurately predicted patient outcomes, with PRKRIP1 expression correlating with immune cell infiltration in the tumor microenvironment 5. Furthermore, novel autoantibodies against PRKRIP1 have been identified as potential biomarkers for chr7 kidney disease, suggesting PRKRIP1's relevance beyond cancer pathology 6.