SLC25A16 is a mitochondrial inner membrane transporter that regulates coenzyme A (CoA) homeostasis between the cytosol and mitochondria. The protein mediates the import of free coenzyme A from the cytosol into the mitochondrial matrix, supporting an enriched mitochondrial CoA pool that enables high-flux tricarboxylic acid cycle and fatty acid oxidation 1. This spatial partitioning of CoA and its thioester derivatives allows independent regulation of mitochondrial energy metabolism and cytosolic lipid synthesis. SLC25A16 variants have been associated with multiple clinical contexts. A genome-wide association study identified a variant in SLC25A16 (rs2394476) with genome-wide significant association to stimulant dependence in individuals of African ancestry 2. The gene has also appeared in studies of cerebral visual impairment as a candidate gene in patients with genetic forms of this condition 3. Additionally, SLC25A16 was identified through Mendelian randomization analysis as a core mitochondrial protein with promising druggability in diabetic nephropathy 4, and longer isoforms show elevated expression in colorectal adenocarcinoma tissues 5. These diverse associations reflect the central role of CoA metabolism in cellular homeostasis across multiple physiological systems.