WBP11 (WW domain binding protein 11) is a core splicing factor that activates pre-mRNA splicing and plays critical roles in development and disease 1. The protein functions as a spliceosome component with the ability to regulate alternative splicing events, including intron retention 2. WBP11 acts through multiple mechanisms: it can competitively bind RNA-binding activities to destabilize specific mRNAs like DUSP16, leading to ERK1/2 activation 3, and it maintains protein stability by inhibiting UFMylation of target proteins such as NONO 4. Developmentally, WBP11 is essential for embryonic viability, as homozygous null mice die before E8.5 1. Heterozygous loss-of-function variants cause multiple congenital defects including vertebral, cardiac, tracheoesophageal, renal, and limb anomalies, contributing to VACTERL association and isolated conditions like Klippel-Feil syndrome 15. In cancer contexts, WBP11 is overexpressed and promotes malignant progression in ovarian cancer, hepatocellular carcinoma, and gastric cancer through various pathways including MCM7 regulation, glycolysis enhancement, and interaction with oncogenic signaling cascades 246. WBP11 has also been identified as a diagnostic biomarker in recurrent pregnancy loss 7.