ZDHHC24 is a palmitoyltransferase that catalyzes S-palmitoylation of diverse protein substrates at the endoplasmic reticulum and Golgi apparatus 1. This enzyme adds palmitate moieties to cysteine residues on target proteins, mediating their membrane anchoring and activation. ZDHHC24 regulates critical cellular processes through palmitoylation of key signaling proteins. It palmitoylates AKT at cysteines 224 and 77, promoting membrane localization and kinase activation independently of PIP3 1. ZDHHC24 also palmitoylates MAVS at the mitochondria, enhancing antiviral innate immunity through the TBK1-IRF3-interferon pathway 2, and palmitoylates B7H3 at cysteine 496, preventing autophagic degradation and supporting immune checkpoint function 3. Additionally, ZDHHC24 catalyzes METTL3 palmitoylation at cysteine 376, promoting condensate formation and protein stability 4, and mediates SRPK1 palmitoylation at multiple cysteines, regulating ferroptosis 5. ZDHHC24 dysfunction associates with multiple diseases. High-fat diets promote hepatocellular carcinoma via ZDHHC24-mediated AKT palmitoylation 1. ZDHHC24 variants are causally linked to generalized anxiety disorder 6 and oral cancer through immune cell-mediated mechanisms 7. ZDHHC24 deletion impairs colorectal cancer immunotherapy resistance 3, suggesting therapeutic potential in immunotherapy and metabolic disease.