ZFAND3 (zinc finger AN1-type containing 3) is a transcriptional regulator with diverse biological functions across multiple cellular contexts. The protein functions as a nuclear transcriptional activator that requires intact zinc-finger domains and nuclear localization for its activity 1. In glioblastoma, ZFAND3 acts within nuclear protein complexes to regulate invasion-related genes including COL6A2, FN1, and NRCAM, critically driving tumor cell invasive capacity 1. ZFAND3 exhibits semi-extractable RNA properties, localizing to nuclear compartments while remaining dissociated from chr6, suggesting involvement in RNA-centric phase separation processes 2. In metabolic contexts, liver-specific ZFAND3 overexpression improves glucose tolerance and insulin resistance, indicating therapeutic potential for lifestyle-related diseases 3. The gene shows significant genetic associations with type 2 diabetes across multiple populations, with variants identified in East Asian genome-wide association studies 4 and replicated in American Indian populations, where sex-specific diabetes associations were observed particularly in women 5. ZFAND3 variants also correlate with tumor volume changes following somatostatin analog treatment in growth hormone-producing pituitary adenomas 6. Additionally, variants have been identified as potential candidates in male infertility, though functional validation studies suggest normal fertility outcomes 7.