ZNF185 is an X-linked zinc finger protein containing a LIM domain that functions as a tumor suppressor and cytoskeletal regulator. The protein localizes to F-actin structures and focal adhesions through its N-terminal actin-targeting domain, while the C-terminal LIM domain is dispensable for cytoskeletal association 1. ZNF185 serves as a direct transcriptional target of both p53 and p63, being upregulated following DNA damage and during epithelial differentiation 23. Functionally, ZNF185 prevents stress fiber formation by inhibiting RhoA activation and promoting endothelial barrier integrity through cAMP/PKA signaling 4. The protein physically interacts with E-cadherin at cell-cell junctions, supporting epithelial differentiation and integrity 3. ZNF185 expression is significantly downregulated in multiple epithelial cancers including prostate, lung adenocarcinoma, and esophageal squamous cell carcinomas, where its loss correlates with tumor progression and metastasis 156. Mechanistically, ZNF185 suppresses cancer cell proliferation and invasion by inhibiting AKT/GSK3β and NOTCH signaling pathways 56. Recent studies have also identified ZNF185 variants in male infertility cases, suggesting a potential role in spermatogenesis 7.