ZWINT (ZW10 interacting kinetochore protein) is a critical component of the outer kinetochore that plays essential roles in chromosome 10 and spindle assembly checkpoint signaling during mitosis. ZWINT functions as part of the KNL1 complex, serving as a docking point for spindle checkpoint components and mediating microtubule-kinetochore interactions 1. The protein is required for proper localization of ZW10 to kinetochores, with ZWINT depletion abolishing ZW10 kinetochore association and leading to aberrant premature chromosome 10 and chromosome 10 formation 1. ZWINT recruitment follows a hierarchical assembly pathway where Hec1 sequentially recruits ZWINT and then ZW10 to kinetochores for faithful chromosome 10 2. The protein's stability is tightly regulated during the cell cycle through APC/C-Cdc20-mediated ubiquitination and degradation in a D-box-dependent manner 3. Clinically, ZWINT overexpression is associated with poor prognosis in multiple cancers, including lung adenocarcinoma, breast cancer, and cervical cancer 456. In cancer contexts, ZWINT promotes cell proliferation, migration, and invasion through various pathways including p53/p21 suppression and p38 MAPK activation, making it a potential therapeutic target 65.