ACTL10 (actin like 10) is a cytoskeletal protein with structural roles in the actin cytoskeleton. The gene is located on chromosome 20 and functions as a structural constituent of the cytoskeleton based on its GO annotations. Regarding mechanism, ACTL10 has been identified in competing endogenous RNA (ceRNA) networks, suggesting it may participate in post-transcriptional regulation through interactions with microRNAs and long non-coding RNAs 1. Disease relevance: ACTL10 has been implicated in cholangiocarcinoma (CCA) as a prognostic mRNA marker within ceRNA regulatory networks 1. Additionally, ACTL10 shows differential DNA methylation patterns in children conceived through assisted reproductive technologies (ART). Specifically, ACTL10-associated CpGs demonstrate hypomethylation in ART-conceived newborns compared to naturally conceived controls 2, and sex-specific methylation differences have been observed, with ACTL10-related CpGs showing hypomethylation in ART-conceived boys 3. Clinical significance: The differential methylation of ACTL10 in ART-conceived offspring may reflect epigenetic modifications related to assisted reproduction, though long-term health implications remain to be determined. Its association with cholangiocarcinoma progression suggests potential diagnostic or prognostic value in liver cancer assessment.