ACYP1 (acylphosphatase 1) is a cytosolic enzyme that catalyzes the hydrolysis of acetyl phosphate to produce acetic acid 1. Beyond its canonical phosphatase activity, ACYP1 functions as a metabolism-related oncogene with pleiotropic roles in cancer progression and cellular metabolism. Mechanistically, ACYP1 promotes glycolysis through the MYC/LDHA axis, with HSP90 playing a critical regulatory role in c-Myc protein stability 2. ACYP1 enhances aerobic glycolysis and the Warburg effect, driving tumor cell proliferation, invasion, and migration 21. Notably, ACYP1 possesses alternative splicing variants with distinct functions; both canonical and CTsv variants induce apoptosis despite CTsv lacking enzymatic activity 3. Clinically, high ACYP1 expression is an independent prognostic factor associated with poor overall survival across hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (iCCA), and combined HCC-iCCA 1. ACYP1 mediates lenvatinib resistance in HCC, and combination targeting of ACYP1 with lenvatinib significantly reduces resistance and tumor progression 2. ACYP1 is also identified in prognostic metabolic gene signatures for HCC, gastric cancer, and acute myocardial infarction 456. Downregulation of ACYP1 reduces cell proliferation, migration, invasiveness, and increases apoptosis in cancer models 1.