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10 sources retrieved · Most recent: April 2026 · Index updated 14 days ago
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ADAM28
ADAM metallopeptidase domain 28
Chromosome 8 · 8p21.2
NCBI Gene: 10863Ensembl: ENSG00000042980.15HGNC: HGNC:206UniProt: A0A384NKT9
53PubMed Papers
20Diseases
0Drugs
0Pathogenic Variants
FUNCTIONAL ROLE
Protease
DATA QUALITY
✓ Experimental GO Evidence✓ Swiss-Prot Reviewed
plasma membraneprotein bindingmetallopeptidase activityimmunoglobulin receptor bindinghypertrophic cardiomyopathyhereditary spastic paraplegiaplacenta praevianeoplasm
✦AI Summary

ADAM28 is a zinc-dependent metalloproteinase with dual protease and adhesion functions that operates distinctly depending on cellular context. In normal tissues, ADAM28 is primarily expressed by epithelial cells in the bronchus, stomach, and epididymis 1, where it protects cells from C1q-induced apoptosis by binding C1q and suppressing cytotoxicity 1. ADAM28 contains a metalloproteinase domain with catalytic activity and a disintegrin domain that interacts with integrins α4β1, α4β7, and α9β1 2. In cancer contexts, ADAM28 exhibits pro-tumoral functions when expressed by carcinoma cells. It is highly overexpressed in lung, breast, and bladder carcinomas 3, where it promotes proliferation, survival, migration, and metastasis through proteolytic cleavage of substrates including insulin-like growth factor binding protein-3 (IGFBP-3), von Willebrand factor (vWF), and connective tissue growth factor (CTGF), while also enhancing PSGL-1/P-selectin-mediated cell adhesion 34. SOX4, an epithelial-mesenchymal transition inducer, directly transactivates ADAM28 expression, suggesting involvement in invasion 5. Conversely, host-derived ADAM28 in the tumor microenvironment exerts protective effects, as ADAM28 knockout mice show accelerated lung colonization and impaired T cell responses 6. Additionally, ADAM28 acts as a novel sheddase of tumor necrosis factor-α and correlates with metabolic syndrome parameters 7.

Sources cited
1
ADAM28 is expressed by epithelial cells in normal tissues and protects from C1q-induced cell death through binding and neutralization of C1q
PMID: 26918856
2
ADAM28 is highly expressed in lung, breast, bladder cancers and chronic lymphocytic leukemia; promotes metastasis through cleavage of vWF, IGFBP-3, CTGF and PSGL-1/P-selectin-mediated adhesion
PMID: 25130845
3
ADAM28 contains metalloproteinase domain with catalytic activity, disintegrin domain interacting with integrins α4β1, α4β7, α9β1; overexpressed in lung and breast carcinomas correlating with proliferation and metastasis
PMID: 19601836
4
ADAM28 from carcinoma cells promotes proliferation and metastasis, while host-derived ADAM28 protects against metastasis and ADAM28 knockout accelerates lung colonization and impairs T cell response
PMID: 32861707
5
SOX4 (EMT inducer) transactivates ADAM28 gene expression and co-localizes with ADAM28 at invasive front of breast and lung carcinomas
PMID: 29882245
6
ADAM28 is multifunctional zinc-dependent metalloproteinase regulating cell adhesion, fusion, membrane protein shedding, and signaling; highly expressed in malignant tumors promoting proliferation, metastasis, and drug resistance
PMID: 40252142
7
ADAM28 is elevated in metabolic syndrome and acts as novel sheddase of TNF-α, with expression correlating with metabolic syndrome parameters
PMID: 23010875
Disease Associationsⓘ20
hypertrophic cardiomyopathyOpen Targets
0.28Weak
hereditary spastic paraplegiaOpen Targets
0.18Weak
placenta praeviaOpen Targets
0.18Weak
neoplasmOpen Targets
0.09Suggestive
non-small cell lung carcinomaOpen Targets
0.09Suggestive
colorectal carcinomaOpen Targets
0.08Suggestive
prostate cancerOpen Targets
0.07Suggestive
benign chondrogenic neoplasmOpen Targets
0.05Suggestive
cancerOpen Targets
0.05Suggestive
prostate carcinomaOpen Targets
0.05Suggestive
Okt4 epitope deficiencyOpen Targets
0.05Suggestive
classic Hodgkin lymphomaOpen Targets
0.04Suggestive
Hodgkins lymphomaOpen Targets
0.04Suggestive
X-Linked Combined Immunodeficiency DiseasesOpen Targets
0.04Suggestive
immunodeficiency 15aOpen Targets
0.04Suggestive
metabolic syndromeOpen Targets
0.04Suggestive
asthmaOpen Targets
0.04Suggestive
macroglobulinemia, Waldenstrom, 1Open Targets
0.04Suggestive
gastric cancerOpen Targets
0.04Suggestive
combined immunodeficiency with skin granulomasOpen Targets
0.03Suggestive
Pathogenic Variants
No pathogenic variants reported on ClinVar for this gene.
View on ClinVar ↗
Related Genes
PRSS21Shared pathway100%LGI1Protein interaction90%ADAM22Protein interaction90%ADGBShared pathway67%ADAM29Shared pathway67%SPMIP7Shared pathway50%
Tissue Expression6 tissues
Bone Marrow
100%
Brain
8%
Lung
7%
Ovary
4%
Heart
3%
Liver
2%
Gene Interaction Network
Click a node to explore
ADAM28PRSS21LGI1ADAM22ADGBADAM29SPMIP7
PROTEIN STRUCTURE
Preparing viewer…
AlphaFoldAI-predicted · UniProt Q9UKQ2
View on AlphaFold ↗
Constraintⓘ
LOEUFⓘ
1.23LoF Tolerant
pLIⓘ
0.00Tolerant
Observed/Expected LoF1.01 [0.84–1.23]
RankingsWhere ADAM28 stands among ~20K protein-coding genes
  • #8,391of 20,598
    Most Researched53
  • #12,950of 17,882
    Most Constrained (LOEUF)1.23
Genes detectedADAM28
Sources retrieved10 papers
Response time—
📄 Sources
10▼
1
ADAM28 is expressed by epithelial cells in human normal tissues and protects from C1q-induced cell death.
PMID: 26918856
FEBS J · 2016
1.00
2
[Research advances on ADAM28 expression and ADAM28-mediated tumor metastasis].
PMID: 25130845
Zhongguo Shi Yan Xue Ye Xue Za Zhi · 2014
0.90
3
ADAM28 as a target for human cancers.
PMID: 19601836
Curr Pharm Des · 2009
0.80
4
ADAM28: Another ambivalent protease in cancer.
PMID: 32861707
Cancer Lett · 2020
0.70
5
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
PMID: 21329817
J Endod · 2011
0.60