AHCTF1 (also known as ELYS/MEL-28) is a nucleoporin essential for nuclear pore complex (NPC) assembly and nuclear envelope formation during mitosis. The protein functions by binding to chr1 through its C-terminal region, specifically through an Arg-Arg-Lys (RRK) stretch that interacts with the nucleosome's acidic patch 1. AHCTF1 serves as an assembly platform that recruits the Nup107-160 subcomplex to initiate post-mitotic NPC formation 2. During mitosis, AHCTF1 undergoes extensive phosphorylation, enabling its interaction with membrane protein VAPB through a phosphorylation-dependent FFAT motif, which facilitates nuclear envelope assembly 3. Beyond structural roles, AHCTF1 participates in gene regulation by mediating oncogenic super-enhancer gating of MYC to nuclear pores, facilitating mRNA export and contributing to cancer cell growth 45. The protein also regulates the phosphorylation state and localization of lamin B receptor (LBR), contributing to nuclear integrity 6. Mutations in AHCTF1 have been identified in follicular thyroid cancer cell lines, suggesting potential therapeutic relevance 7. The gene has also been associated with coagulation factor IX activity regulation 8.