AMPH (amphiphysin) is a protein that participates in regulated exocytosis at synapses and endocrine cells, functioning as a key regulator of synaptic vesicle dynamics and membrane-associated cytoskeleton properties. The protein localizes to synaptic vesicle membranes and plasma membranes, where it mediates synaptic vesicle endocytosis through interactions with phospholipids and binding partners 1. AMPH controls actin cytoskeleton organization at the leading edge and within the cytoplasm, essential for vesicular trafficking mechanisms. Its primary mechanism involves coordinating membrane curvature and cytoskeletal remodeling during endocytotic processes, supporting both chemical synaptic transmission and regulated secretion in specialized cell types. Disease associations indicate AMPH dysfunction contributes to developmental and epileptic encephalopathy-9 and Dravet syndrome, reflecting its critical role in neuronal function 2. The protein's involvement in synaptic vesicle dynamics suggests broader relevance to neurological disorders affecting synaptic plasticity and neurotransmitter release. Additionally, AMPH-related molecular innovations have enabled therapeutic developments, including amphiphile-based vaccine technologies that enhance immune targeting 345. These applications leverage amphiphilic properties for lymph node delivery and cellular targeting, demonstrating translational significance beyond its endogenous synaptic functions.