ANKRD49 (ankyrin repeat domain 49) is a transcriptional regulator that induces HBG1 expression 12 and functions as a protein-binding domain protein involved in positive regulation of DNA-templated transcription. In spermatogenesis, ANKRD49 promotes autophagy via the NF-κB pathway and inhibits intrinsic apoptosis in germ cells by modulating NF-κB signaling 3. Clinically, ANKRD49 is significantly elevated across multiple carcinomas and serves as an independent prognostic indicator. In lung adenocarcinoma, ANKRD49 promotes invasion and metastasis through upregulation of matrix metalloproteinase-2 and -9 via the P38/ATF-2 signaling pathway 4 and facilitates epithelial-mesenchymal transition by interacting with PKNOX1 to activate the TGF-β1/SMAD pathway 5. In lung squamous cell carcinoma, elevated ANKRD49 correlates with lymph node metastasis and poor overall survival 6. Similarly, in glioma 7 and gastric cancer 8, high ANKRD49 expression is associated with advanced disease grade, increased proliferation, and poor prognosis. ANKRD49 represents a potential therapeutic target and biomarker for cancer patient stratification and treatment selection.