ANKS4B (ankyrin repeat and sterile alpha motif domain containing 4B) serves as a critical scaffolding protein essential for intermicrovillar adhesion complex formation in epithelial brush borders 1. The protein localizes to the tips of adherent brush border microvilli and is required for intermicrovillar adhesion, playing a fundamental role in brush border assembly and microvilli organization 1. Mechanistically, ANKS4B functions as part of a tripartite complex with USH1C and MYO7B, where it binds to the MYO7B cargo-binding tail at distinct sites from USH1C 1. Complex formation follows a specific hierarchy where ANKS4B and MYO7B must first be activated by USH1C before the stable ternary complex can assemble 12. This complex anchors protocadherin-based tip-links that connect adjacent microvilli, linking cadherins to the actin cytoskeleton 23. The MYO7B/USH1C/ANKS4B complex can form condensed molecular assemblies via liquid-liquid phase separation, which may contribute to structural organization 4. Additionally, ANKS4B has been identified as a target of HNF4α in pancreatic β-cells, where it regulates cellular susceptibility to endoplasmic reticulum stress 5. Disease relevance includes potential involvement in gut and kidney diseases affecting brush border structures and pancreatic β-cell dysfunction 25.