ARHGAP24 (Rho GTPase activating protein 24) is a tumor suppressor that functions as a GTPase-activating protein primarily targeting Rac-type GTPases 1. The protein contains a pleckstrin homology (PH) domain and C-terminal coiled-coil domain, belonging to a RhoGAP subfamily 1. ARHGAP24 regulates cell proliferation, migration, and invasion through both GTPase-dependent and GTPase-independent mechanisms. In its canonical role, ARHGAP24 inactivates RhoA and Rac1 GTPases, leading to inhibition of cell proliferation and invasion 23. However, recent evidence demonstrates that ARHGAP24 also functions as a GTPase-independent scaffold protein, recruiting the E3 ubiquitin ligase WWP1 to promote degradation of pyruvate kinase M2 (PKM2), thereby suppressing β-catenin transactivation 4. ARHGAP24 expression is significantly downregulated in multiple cancer types including hepatocellular carcinoma, lung cancer, colorectal cancer, and renal cell carcinoma, where low expression correlates with poor prognosis 4253. Additionally, ARHGAP24 deletion has been identified as a concurrent alteration with 13q14 deletion in B-cell chr4 lymphocytic leukemia 6. The protein also plays a role in restricting HIV-1 entry into dendritic cells through its involvement in endocytosis regulation 7.