ARL14 (ARF like GTPase 14) is a small GTPase that plays a critical role in MHC class II antigen presentation by controlling vesicle transport in dendritic cells. The primary function of ARL14 is to recruit the motor protein MYO1E to MHC class II-containing vesicles through its effector protein ARL14EP, thereby controlling the movement of these vesicles along the actin cytoskeleton 1. The protein's subcellular localization is determined by its N-terminal amphipathic helix, which targets it to endosomes and plasma membrane in a GTP-independent manner 2. ARL14 exhibits tissue-specific expression, being confined to the stomach and intestines 3. Beyond immune function, ARL14 can activate phospholipase D1 and is involved in cargo trafficking via the ESCPE-1 complex 3. Disease relevance includes associations with severe malaria susceptibility, where regulatory variants in the ARL14 promoter influence gene expression and disease risk 4. Additionally, ARL14 has been identified as a potential prognostic biomarker in non-small cell lung cancer, with knockdown experiments showing inhibition of malignant cell phenotypes 5. The gene shows dynamic splicing patterns and sex-specific expression differences across tissues 6.