ASAP1 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 1) is a multifunctional protein that primarily serves as a GTPase-activating protein for ADP-ribosylation factors (ARFs), particularly ARF1, ARF4, and ARF5 1. The protein plays critical roles in multiple cellular processes including cytoskeletal regulation, membrane trafficking, and cell migration. ASAP1 directly interacts with molecular motors including myosins and kinesins, influencing actin cytoskeleton remodeling and microtubule dynamics essential for cell movement 2. In gastric cancer, ASAP1 promotes tumor progression and chemotherapy resistance by inhibiting ubiquitin-mediated degradation of IQGAP1, thereby enhancing CDC42 activity and upregulating the EGFR-MAPK pathway 3. The protein also functions in membrane trafficking through interaction with ARF4, where it can regulate endothelial cell survival in diabetic kidney disease 1. Additionally, ASAP1 has been identified as a potential biomarker in various contexts, including intervertebral disc degeneration and melanoma targeting 45. However, genetic variants in ASAP1 showed no significant association with tuberculosis susceptibility in Chinese populations 6.