ASB5 (ankyrin repeat and SOCS box containing 5) is an E3 ubiquitin ligase that functions as a substrate-recognition component of SCF-like ECS complexes, mediating ubiquitination and proteasomal degradation of target proteins 1. The six-ankyrin repeat domain-containing ASB proteins, including ASB5, display strong evolutionary conservation and regulate compartment size expansion, with mechanistic roles involving canonical Notch signaling and mitochondrial function regulation 1. ASB5 serves as a specific and abundantly expressed marker of muscle satellite cells (MuSCs) and myogenic progenitors 2. Despite its prominent expression in skeletal muscle, CRISPR-generated Asb5 knockout mice develop normally with unimpaired postnatal growth, MuSC proliferation, differentiation, and skeletal muscle regeneration after acute injury 2. The knockout reduces TNFα expression levels in skeletal muscle 2. Beyond muscle, ASB5 shows disease relevance in incisional hernias, where it was identified as a key differentially expressed gene associated with extracellular matrix imbalance and inflammatory responses 3. ASB5 appears in early-stage gastric cancer prognostic signatures as an overexpressed gene in high-risk, short-survival patient groups 4. Additionally, ASB5 associates with essential and polyunsaturated fatty acid metabolism in beef cattle muscle [PMID:35384007; 55]. Its tissue-specific epigenetic regulation in skeletal muscle involves super-enhancers and promoter DNA hypomethylation patterns 6.