ASPDH (aspartate dehydrogenase domain containing) is a gene located on chromosome 19 whose protein product exhibits enzymatic activity characteristic of aspartate dehydrogenases. The ASPDH enzyme demonstrates NADH-dependent reductive activity toward oxaloacetate and other 2-keto acids, with catalytic parameters indicating Km values of 4.25 mM for oxaloacetate 1. The enzyme's activity is modulated by metal ions, with Ca2+ and Fe2+ enhancing activity while Cu2+ and EDTA inhibit it 1. Beyond its metabolic function, ASPDH has been identified as a less selective binding partner for NAADP, a potent calcium-mobilizing second messenger 2, potentially linking it to calcium signaling pathways implicated in cancer and immunity. In clinical contexts, ASPDH has emerged as a candidate gene in hepatocellular carcinoma research, identified among fatty acid metabolism-related genes associated with diagnostic and prognostic significance 3. Additionally, ASPDH methylation patterns in sperm have been associated with reproductive traits in cattle, suggesting epigenomic regulation may influence phenotypic outcomes 4. These findings indicate ASPDH functions in metabolic pathways while potentially participating in signaling mechanisms relevant to disease processes, though comprehensive characterization of its specific biological role in human cells remains limited.