AUNIP (Aurora Kinase A and Ninein Interacting Protein) is a DNA-binding protein that functions as a critical sensor and regulator of DNA double-strand break (DSB) repair pathway choice. AUNIP accumulates at DSB sites following DNA damage and possesses intrinsic DNA-binding ability with strong preference for DNA substrates mimicking stalled replication fork structures 1. Mechanistically, AUNIP recruits and anchors RBBP8/CtIP to DSBs, promoting DNA end resection and homologous recombination (HR) repair while simultaneously inhibiting non-homologous end joining (NHEJ) 1. During the cell cycle, AUNIP is required for dynamic movement of aurora kinase A at centrosomes and the spindle apparatus, contributing to spindle organization and proper cell cycle progression 2. Clinically, AUNIP dysregulation has emerged as relevant to multiple diseases. In oral squamous cell carcinoma (OSCC), AUNIP expression increases with malignant differentiation, and its suppression inhibits cancer cell proliferation and induces G0/G1 arrest; elevated AUNIP predicts poor prognosis 3. In lung adenocarcinoma, AUNIP functions as an oncogene promoting cell proliferation and correlates with immune infiltration and patient outcomes 4. Additionally, rare AUNIP variants were identified as potential risk associations for Parkinson's disease in large-scale exome analysis, though replication across independent datasets was unsuccessful 5. These findings position AUNIP as both a fundamental DNA repair regulator and a promising biomarker for cancer prognosis and potential therapeutic target.